In Ethiopia, particularly in the Tana Sub-Basin, irrigation development practice is increasing. However, this development ignored the fisheries; no, enough information about its effects. The sub-basin is rich in fisheries, including the 17 Labeobarbus species (the only remaining cyprinid species flock in the world). The fishery also supports over 6,000 fishers. Hence, this study investigated the impact of irrigation practices on the Gilgel Abay, Ribb, and Gumara fisheries. Methods include fish sampling below and above the weirs; expert interviews; key informant interviews; secondary data; and impact significance matrix methods. The data collection time was from July 2019 to June 2020. The analysis of the data was qualitative and quantitative. The existing irrigation system affects fisheries by blocking upstream spawning migration routes (Gilgel Abay Weir and Ribb Dam, for sure catch below the Gilgel Abay Weir, significantly higher than above the weir, Shannon Index (H’), P< 0.001). Besides, according to local sources, after 2007, Gumara and Ribb rivers became seasonal because of excessive water abstraction for irrigation, resulting in mass fish-killing and the failure of juvenile recruitment to the lake. In one instance, we recorded the deaths of over 930 adults and juveniles on the Gumara and the Ribb rivers. The other problem is that, due to low water volume, even non-fishers collect fish from the pools; and during spawning time, fishers target spawning migratory species at the weirs where the catch is prime. Other threatening elements can also aggravate the impact. Hence, these impacts need to be ameliorated by practicing efficacious water use, catchment treatment, fishery management, fish ladder development, and factor alleviation can be solutions.